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In another misunderstanding in Mexico, conquering Spaniards first reached the eastern shore of a vast Gulf peninsula in the early 1500s. Striking inland they asked the Mayan natives they encountered what the name of the place was. Repetitions of the question did not help Mayans, not speaking 16th Century Castilian, replied with terms that meant don understand or don know The Spaniards, in their smug belief they were communicating, thought they had been given an answer in an oft heard response: ( don know They named the peninsula that; they also dubbed the people of the land by that name as well.
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In batch culture fermentation systems inoculated with human faeces, growth of Enterobacteriaceae was stimulated in the presence of TMAO. Human derived faecal and caecal bacteria (n=66 isolates) were screened on solid and liquid media for their ability to use TMAO, with metabolites in spent media analysed by 1H NMR. As with the in vitro fermentation experiments, TMAO stimulated the growth of Enterobacteriaceae; these bacteria produced most TMA from TMAO.
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